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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by <t>ELISA</t> after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
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Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by ELISA after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.

Journal: bioRxiv

Article Title: Phospotyrosine proteomics reveals novel Zap70 and Itk pathway targets downstream of TCR and CAR in Jurkat T cells

doi: 10.1101/2025.11.03.686362

Figure Lengend Snippet: Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by ELISA after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.

Article Snippet: Interleuken-2 abundance was assessed using the Human IL-2 Sandwich ELISA Kit (proteintech #KE00017) per the manufacturer’s instructions.

Techniques: Expressing, Flow Cytometry, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Co-Culture Assay